Coding

Part:BBa_K775000:Design

Designed by: Sietske Grijseels   Group: iGEM12_TU-Delft   (2012-08-31)

RI7-GPR109A Niacin chimeric GPCR


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 582
    Illegal BamHI site found at 113
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 478


Design Notes

Promoter: GPD Terminator: CYC1.


Source

N-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat) Ligand binding domain: G protein-coupled receptor 109A EDM13619.1, Rattus norvegicus (Rat) C-terminus: Olfactory receptor-like protein I7 (olr226), Rattus norvegicus (Rat)

References